Conducting TIER I laboratory studies & complex higher tier studies

These are conducted under extended laboratory, semi-field and field conditions with standard or special designs. All studies are planned and performed by a highly experienced team of scientists and technical personnel according to the recent guidelines (IOBC, OECD, ISO, EPA) and guidance documents. 

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Honey Bees, Bumble Bees and Solitary Bees

World leaders in testing the effects of plant protection products (PPPs) on honey bees (Apis mellifera) and bumble bees (Bombus terrestris).

Our experts follow OECD Guidelines for laboratory studies and OPPTS guidelines for extended studies and have been actively involved in multiple international ring test groups. This activity has led to several OECD guidelines and guidance documents on laboratory testing methods.

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Honey Bee (Apis mellifera):

  • Laboratory Acute Toxicity Test: OECD 213 / OECD 214
  • Laboratory Chronic Toxicity Test:  OECD 245
  • Larval Toxicity Test:  OECD 237 and OECD guidance document 239
  • Extended Laboratory Test:  OPPTS 850.3030
  • Honey Bee Semi-Field and Field Tests: OECD 75, EPPO 170, CEB 230
  • Honey Bee Brood Feeding Study: EPPO (Oomen et al. (1992 a+b)), EFSA Guideline

Bumble bee (Bombus terrestris):

  • Laboratory Acute Toxicity Test: OECD 246 / OECD 247
  • Laboratory Chronic Toxicity Test: based on OECD 245 and OECD 247
  • Bumble Bee Semi-Field and Field Tests: OECD 75, EPPO 170, CEB 230
  • Coated seeds effect of dust and of guttation

Solitary bee (Osmia bicornis):

  • Laboratory Acute Toxicity Test
  • Solitary bee Semi-Field and Field Tests

Honey Bee Stomach and Pollen Residue Studies

Sampling of nectar, pollen and hive matrices (wax, pollen (bee bread), nectar / honey) can provide information to evaluate possible risks to pollinators from residual traces of plant protection products. The exposure of pollinators to potential residues is also possible via soil, plant matrices, water (vegetative plant material, flowers, guttation liquid) and dust generated during drilling of coated seeds. 

To determine the amount of residues carried into the hive by forager bees after PPP application, honey stomach preparations are performed. Deep frozen samples of honey bees collected at intervals after application are sent to our laboratory where the bees are dissected for the manual extraction of their honey stomach contents. The pollen loads can also be collected for analysis. The obtained samples can be either analysed at our facility or stored until shipment to the selected analytical laboratory.

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Non-Target Arthropods

We offer a wide range of standardized laboratory studies to determine the ecotoxicological effects of plant protection products on non-target arthropods. 

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Studies can be conducted with leaf dwelling organisms such as parasitic wasps (Aphidius rhopalosiphi, Trichogramma cacoeciae), predatory mites (Typhlodromus pyri), aphidophagous predators (Chrysoperla carnea, Coccinella septempunctata) and anthocorid bugs (Orius laevigatus). Furthermore we offer studies with soil dwelling organisms like the carabid species Poecilus cupreus (adults and larvae), the staphylinid beetle Aleochara bilineata, and the lycosid spider genus Pardosa.

  • Tier I: Laboratory Studies: IOBC/WPRS 2000
  • Tier II: Extended Laboratory Studies: IOBC/WPRS 2000 or IOBC 2009
  • Higher Tier: Aged Residue Studies: based on IOBC/WPRS 2000 and Semi Field Studies
  • Field study: Predatory mite field studies, NTA Field In and Off-Crop, Arthropod insect residue and drift residue field.

We also customize non-standard studies for specific issues. For example, 

  • Effects of granulated test items on Aphidius rhopalosiphi (laboratory dust studies)
  • Wind tunnel studies to mimic spray drift exposure
  • Testing of coated seed or metabolites in laboratory and higher tier studies with soil inhabiting arthropods
  • The sexually reproducing Sinella curviseta can be tested as an alternative to the parthenogenetic collembolan species Folsomia candida 
  • Testing of the oribatid mite Oppia nitens (ISO Draft 23226:2018)

Furthermore, our group is involved in new test system development( e.g. with herbivorous butterfly larvae (Vanessa cardui and Pierris brassicae) feeding on test item treated leaves (exposure via food uptake) to address relevant requirements of current EFSA opinion papers.

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Non-Target Soil Organisms

Our experienced teams conduct ecotoxicological acute and chronic studies with soil organisms.

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Our teams have more than 25 years experience in laboratory and field testing of ecotoxicological effects on earthworms, springtails and predatory mites. 

This includes earthworms, micro-arthropods (springtails and mites) and soil microorganisms. In addition to the standard application, coated seeds, pellets, granules with formulations, actives and metabolites are tested using modified test designs. If required, verification of application can be linked to in-house residue analysis.

Earthworms (Eisenia fetida/andrei):

  • Acute toxicity test: OECD 207 / ISO 11268-1
  • Reproduction test: OECD 222 / ISO 11268-2
  • Bioaccumulation study: OECD 317

Enchytraeids (Enchytraeus albidus/crypticus): 

  • Reproduction test: OECD 222 

Soil Micro-Arthropods:

  • Springtails (Folsomia candida) - Reproduction test: OECD 232
  • Predatory mites (Hypoaspis aculeifer) - Reproduction test: OECD 226

Soil Microflora:

  • Nitrogen Transformation Test: OECD 216
  • Short-Term Respiration Test: OECD 217

Mycorrhizal fungi (Glomus mossae): 

  • Spore germination test: ISO 10832

Field studies:

Semi-field and field tests (litterbag, earthworm field trials and micro-arthropod trials) can be performed throughout Europe, with soil organisms including earthworms, nematodes, several soil inhabiting arthropod species including micro-arthropods (collembolans and soil mites)..

We also customize non-standard studies for specific issues or species, for example:

  • Drip irrigation system under semi-field conditions
  • Testing with endogeic earthworm species Aporrectodea caliginosa
  • Tests on a set of natural soils

Furthermore, our group is involved in new test system development( e.g. terrestrial isopod (Porcellio scaber) feeding on test item treated leaf litter) to address relevant requirements of current EFSA opinion papers.

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Non-Target Terrestrial Plants

Evaluate the potential effects of your plant protection product on emergence and vegetative vigour of plant seedlings.

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We perform tests on seedling emergence and seedling early growth, observing the germination and growth of plant seeds or to evaluate the potential effects on the above-ground portions of plants. 

Additionally, we can conduct tests using treated soil which are designed to determine the inhibition of growth and reproductive capability of higher plants according to ISO 22030. In accordance with OECD or OCSPP Guidelines, tests are performed under defined conditions in a greenhouse so these studies can be conducted all year round.

  • Seedling Emergence Test: OECD 208 / OCSPP 850.4100
  • Vegetative Vigour Test: OECD 227 / OCSPP 850.4150
  • Soil Quality - Biological Methods - Chronic Toxicity in Higher Plants - ISO 22030 

We also conduct customized studies with Non-Target Terrestrial Plants such as:

  • Semi-field studies under a UV-permeable roof with rain protection
  • Testing non-crop plant species at the vegetative and generative growth stage

Furthermore, our group is involved in new test system development for (the testing of non-crop plant species and generative endpoints to address relevant requirements of current EFSA opinion papers.

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Non-Target Dung Organisms

We conduct studies for the testing of veterinary pharmaceuticals with dung organisms including the dung fly species Musca autumnalis and Scatophaga stercoraria, and the dung beetles Onthophagus taurus and Aphodius constans

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This includes studies with the dung fly species Musca autumnalis and Scatophaga stercoraria and the dung beetles Onthophagus taurus and Aphodius constans. In these studies, the test organisms are exposed to the test substance mixed with cattle dung obtained from organic farming. We are members of the DOTTs group and have been involved in the development of the test guideline for dung flies and in the ring test with O. taurus.

  • Dung Flies (Scathophaga stercoraria, Musca autumnalis): Developmental toxicity test: OECD 228
  • Dung beetles (Aphodius constans, Onthophagus taurus): Developmental toxicity test: OECD Guidance 122
  • Reproduction toxicity test – according to the revised version of the ring test protocol
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Avian Safety Services

The world’s premier avian safety laboratory.

Design and conduct of customized acute reproduction and safety assessment studies to evaluate pesticide hazard to avian species.

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Established in 1985, our Easton, Maryland facility offer the full suite of required acute and reproduction studies (radiolabeled and unlabeled), as well as custom-designed investigations in non-traditional species or to meet client-specific needs.

  • Acute oral safety assessments – including regulatory endpoints
  • Subacute dietary safety assessments – including passerines
  • Pathogenicity and safety studies for the testing of biopesticides
  • Food avoidance/repellency studies with treated seeds and granules
  • Reproduction studies (over 500 conducted)
  • Adjusted designs to address specific risk assessment issues
  • Studies to screen and/or test for potential endocrine effects
  • Multi-generation reproduction studies with endocrine endpoints
  • Hen delayed neurotoxicity studies
  • Poultry feeding and metabolism studies
  • Residue and metabolism studies in avian species
  • Chemistry support
  • Custom-designed studies

In addition to commonly tested species such as northern bobwhite, quail, Japanese quail (Coturnix), mallard, zebra finch and canary, we have deep experience working with a variety of domestic and wild species, including chickens, pheasants, house sparrows, pigeons and partridge.

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Other field studies and additional services

Standard and custom studies to fulfil the environmental risk assessment. 

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Additional field studies:

  • Pollinator monitoring studies focus on native bee communities occurring in agroecosystems and are performed under field conditions 
  • Drift Residue laboratory and Field Studies investigate the dust deposition during sowing of coated seeds or granule application with pneumatic or mechanical sowing machinery. Field studies are carried out following the draft Guideline SANCO/10553/2012 rev. 16 (2018).
  • Birds or mammals monitoring studies aim at obtaining generic data and determining the potential impact of plant protection products (PPPs) under conditions of realistic use.

Customized non-standard studies:

  • Testing of coated seed or metabolites in laboratory and higher tier studies with soil inhabiting arthropods
  • Drip irrigation system under semi-field conditions)
  • Testing of the endogeic earthworm species Aporrectodea caliginosa in natural soil
  • Laboratory dust studies with granulated test items
  • Wind tunnel studies to mimic spray drift exposure
  • NTTP semi-field studies under a UV-permeable roof with rain protection
  • Testing of non-crop terrestrial plant species at the vegetative and generative growth stage
  • Non-Target In-Soil Organisms can be tested with a set of natural soils
  • As an alternative to the parthenogenetic collebolan species Folsomia candida the sexually reproducing Sinella curviseta can be tested
  • Testing of the oribatid mite Oppia nitens (ISO Draft 23226:2018)

Non-GLP screening-tests with:

Eisenia fetida, Folsomia candida, Hypoaspis aculeifer, Typhldoromus pyri, Aphidius rhopalosiphi, Apis mellifera and Bombus terrestris

Test system development:

  • Terrestrial isopods (Porcellio scaber) feeding on test item treated leaf litter (exposure via food uptake)
  • Herbivorous butterfly larvae (Vanessa cardui and Pierris brassicae) feeding on test item treated leaves (exposure via food uptake)
  • Tests with Apis mellifera in laboratory in autumn and winter in Spain